KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0254 | - |
versiconal hemiacetal acetate | isoform VHA reductase II, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C | Aspergillus parasiticus | |
0.0354 | - |
versiconal hemiacetal acetate | isoform VHA reductase I, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C | Aspergillus parasiticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Aspergillus parasiticus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
10 * 39000, isoform VHA reductase I, SDS-PAGE | Aspergillus parasiticus |
40000 | - |
10 * 40000, isoform VHA reductase II, SDS-PAGE | Aspergillus parasiticus |
390000 | - |
gel filtration | Aspergillus parasiticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
versiconal hemiacetal acetate + NADPH + H+ | Aspergillus parasiticus | both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced | versiconol acetate + NADP+ | - |
r | |
versiconal hemiacetal acetate + NADPH + H+ | Aspergillus parasiticus NUIH-26 | both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced | versiconol acetate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus parasiticus | - |
- |
- |
Aspergillus parasiticus NUIH-26 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, Sephacryl S-300 gel filtration, hydroxylapatite column chromatography, and Matrex gel Green A chromatography | Aspergillus parasiticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus parasiticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00362 | - |
crude extract, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C | Aspergillus parasiticus |
0.027 | - |
isoform VHA reductase II, after 7.5fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C | Aspergillus parasiticus |
0.052 | - |
isoform VHA reductase I, after 14.4fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C | Aspergillus parasiticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
versiconal hemiacetal acetate + NADPH + H+ | both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced | Aspergillus parasiticus | versiconol acetate + NADP+ | - |
r | |
versiconal hemiacetal acetate + NADPH + H+ | both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced | Aspergillus parasiticus NUIH-26 | versiconol acetate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodecamer | 10 * 39000, isoform VHA reductase I, SDS-PAGE | Aspergillus parasiticus |
homodecamer | 10 * 40000, isoform VHA reductase II, SDS-PAGE | Aspergillus parasiticus |
Synonyms | Comment | Organism |
---|---|---|
VHA reductase I | - |
Aspergillus parasiticus |
VHA reductase II | - |
Aspergillus parasiticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
isoform VHA reductase I | Aspergillus parasiticus |
9 | - |
isoform VHA reductase II | Aspergillus parasiticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | no activity is detected below pH 4.0 | Aspergillus parasiticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Aspergillus parasiticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus parasiticus | isoform VHA reductase II, isoelectric focusing | - |
6 |
Aspergillus parasiticus | isoform VHA reductase I, isoelectric focusing | - |
6.6 |